ok cells  (CLS Cell Lines Service GmbH)

Journal: Cell communication and signaling : CCS

Article Title: STAT3 blockade ameliorates LPS-induced kidney injury through macrophage-driven inflammation.

doi: 10.1186/s12964-024-01841-1

Figure Lengend Snippet: Fig. 6 Inhibition of STAT3 attenuates tubular epithelial cell injury in a co-culture system with macrophages. A A schematic illustration of co-culture system with macrophages and tubular epithelial cells. B Western blot results of IL-6, KIM-1, pSTAT3, and STAT3 expression in RAW264.7 and OK cells are shown. C Upregulated expression of KIM-1 in OK cells co-cultured with LPS-stimulated RAW264.7 cells significantly decreased upon pre- and concomitant-treatment of 2 μM Stattic. All experiments were independently replicated at least three times, and the data are presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001

Article Snippet: The murine macrophage cell line RAW264.7 (TIB-71) and the proximal tubule epithelial cell line OK (CRL1840) were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Inhibition, Co-Culture Assay, Western Blot, Expressing, Cell Culture